laser speckle perfusion system  (Perimed Inc)

Journal: Bioactive Materials

Article Title: Reconstructing the ischemic osteogenic microenvironment through hierarchical scaffolds orchestrating Mg 2+ signaling and neuropilin-1–mediated angiogenesis

doi: 10.1016/j.bioactmat.2026.02.031

Figure Lengend Snippet: Imaging evaluation in animal experiments. ( A ) Schematic diagram of animal experiments. ( B ) Pre- and postoperative speckle contrast imaging of the periosteum at the lateral femoral condyle. ( C ) X-ray and CT images of the bone defect repair site at 6 and 12 weeks postoperatively (green indicates the implanted scaffold; gray indicates the surrounding newly formed bone). ( D-G ) Quantitative analysis of BV/TV, Tb.N, Tb. Sp, Tb.Th. Data are presented as mean values ± s.d. (n = 3). ( H ) Laser speckle contrast images of the bone defect repair site in different groups at 6 and 12 weeks postoperatively. ( I-J ) Quantitative analysis of vessel intensity and perfusion integrated density. Data are presented as mean values ± s.d. (n = 3). ns, no significant (p > 0.05), ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).

Article Snippet: Under isoflurane anesthesia, the lateral knee region was surgically exposed and imaged using a laser speckle perfusion system (PeriCam PSI System, Perimed AB, Sweden; 785 nm).

Techniques: Imaging

Journal: Molecular Therapy

Article Title: Tissue nanotransfection-based endothelial PLCγ2-targeted epigenetic gene editing rescues perfusion and diabetic ischemic wound healing

doi: 10.1016/j.ymthe.2025.01.034

Figure Lengend Snippet: Endothelial-targeted demethylation of Plcγ2 improves VEGF therapy on diabetic wound-edge vascularization (A) Vector components used for targeted demethylation of Plcγ2 promoter in STZ-induced diabetic mice using CRISPR-dCas9 approach. Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed laser speckle-assisted perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units. TNT procedure was done with scramble-gRNA, VEGF only, demethylation cocktail only, or VEGF plus demethylation cocktail CDH5 promoter-driven (endothelial) Plcγ2 gRNA in presence of dCas9-TET1CD cocktail. Perfusion was calculated based on the ratio of the wound area vs. distant normal skin. n = 6–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 + VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 + VEGF vs. VEGF (two-way ANOVA, followed by Tukey’s HSD post hoc test). (F) Blood flow and pulse pressure at the wound edge were measured using the pulse wave Doppler feature of Vevo 2100. The pulse wave profiles at d10 are shown (left). The increases in blood flow and pulsation as indicated by increase in peaks heights are shown for the respective groups compared to the control. The percentage of VTI and the calculated pulse pressure are shown as bar graphs (right). n = 6, ∗ p < 0.05 (one-way ANOVA, followed by Tukey’s HSD post hoc test). VTI, velocity time integral; scr gRNA, scramble-gRNA; VEGF, VEGF ORFs; gRNA PLCγ2 , gRNAs for Plcγ2 promoter; STZ, streptozotocin. (G) Wound closure was monitored at different days after wounding in abovementioned murine bipedicle ischemic wounds (D) subjected to TNT by digital planimetry (scale bar, 5mm) (H). n = 5–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 +VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 +VEGF vs. VEGF (two-way ANOVA, followed by Tukey HSD post hoc test).

Article Snippet: Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed laser speckle-assisted perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units.

Techniques: Plasmid Preparation, CRISPR, Control